Immunofluorescent Staining: the Fluorescent Antibody Method.

I. Principles of Tmmunofluorescent Staining.................................................. A. Fluorescent Antibody Labeling........................................................ B. Immunofluorescent Staining Procedures............................................... C. Controls.............................................................................. D. Nonspecific Staining.................................................................. II. Antibody Synthesis and Reactions in Vivo................................................. A. Synthesis............................................................................. B. Hypersensitivity Reactions............................................................ III. Immunofluorescent Staining of Microorganisms .......................................... A. Viruses............................................................................... B. Bacteria, Protozoa, and Fungi.......................................................... IV. Immunofluorescent Staining of Tissue Antigens........................................... A. Heteroantibodies to Tissue Antigens................................................... B. Isoantibodies. .. .......................................................................